Design precise editing of genomic DNA by homology-directed repair (HDR)
Step1: Design guides that target mutation site
Choose your organism:
Solanum lycopersicum
"
Solanum tuberosum
"
Lactuca sativa
"
Daucus carrota
"
Theobroma cacao
"
Pisum sativum
"
Cucumis sativus
"
Capsicum annuum
"
Plantago ovata
"
Spinacia oleracea
"
Mentha longifolia
"
Eruca vesicaria
"
Ocimum basilicum
"
Salvia rosmarinus
"
Brassica oleraceacapitata
"
Brassica oleraceaacephala
"
What region are you interested in:
Promoter
"
terminator
"
CDS
"
Enter a gene ID:
Enter at least 10 nucleotides that span the target mutation
Submit
Step2: design point mutation
Wild type codon
Desired amino acid:
A
"
C
"
D
"
E
"
F
"
G
"
H
"
I
"
K
"
L
"
M
"
N
"
P
"
Q
"
R
"
S
"
T
"
V
"
W
"
Y
"
Submit
Step3: Design DS donor DNA that displays target mutations
Choose your organism:
Solanum lycopersicum
"
Solanum tuberosum
"
Lactuca sativa
"
Daucus carrota
"
Theobroma cacao
"
Pisum sativum
"
Cucumis sativus
"
Capsicum annuum
"
Plantago ovata
"
Spinacia oleracea
"
Mentha longifolia
"
Eruca vesicaria
"
Ocimum basilicum
"
Salvia rosmarinus
"
Brassica oleraceacapitata
"
Brassica oleraceaacephala
"
What region are you interested in:
Promoter
"
terminator
"
CDS
"
Enter a gene ID:
Enter at least 10 nucleotides that span the target mutation
Enter WT sequence (at least 10 nt).
Enter multiple sequences separated by a coma.The sequences must not overlap.
Enter the same sequence as above with modifications.
The multiple sequences must be in the same order as above
Enter the length of the desired left arm of the donor (recommended 500 nt)
Enter the length of the desired right arm of the donor (recommended 500 nt)
Submit
-->